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Moreover, an inverse relation between the hardness and the tribological performance was noted, i.e. the sintered BMG had stronger wear resistance than the other samples for long distance sliding.
Competent cells obtained were immediately used or conserved at −80°C as 100 µl samples for long term storage.
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All participants provided written informed consent and consented serum samples for long-term storage [ 21].
Remaining samples for long-term storage (whole blood, serum, plasma, urine and saliva samples) will be kept at −70° to 80° Celsius at the Biobank Institute of the Medical University of Graz.
For our analysis, we selected 16 samples of animals with optimal nutrition: 8 samples for normal specimen (four at each time point) and 8 samples for long-lived specimen (four at each time point).
They also cautioned that a combination of the MSAO with the MQBO may also cause such asymmetry if the time series is not sampled for long enough.
Fluorescent antibodies are highly specific, but it is difficult to get them close to the structure of interest, as they are quite large and cannot diffuse through a biological tissue sample for long distances.
Using statistical power as criterion, the results demonstrated quantitatively the consequences of various sampling strategies, and could guide users with respect of required sample sizes depending on sampling design for long term monitoring programs.
In addition, the samples collected for long term storage have been systematically managed with the BSRS (Biospecimen Storage and Requisition System).
To identify a spectrum of mutations, after long-term propagation, we combined whole genome sequencing (WGS) and oligonucleotide array Comparative Genomic Hybridization (oaCGH) analysis of a C. elegans strain that was propagated, in the absence of DOG-1 and MDF-1/MAD1, for a total of 470 generations, with samples taken for long term storage (by freezing) in generations 170 and 270.
A collection of CSF samples requires lumbar puncture, a relatively risky and uncomfortable process which is therefore unsuitable for large-scale screening or repeated sampling for long-term monitoring of disease progression.
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