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Tissue samples for light microscopy were fixed in 4% formaldehyde and embedded in paraffin using routine procedures.
All human brain samples for light microscopy were obtained from the brain bank at the Institute for Clinical Neuroanatomy, University of Frankfurt (Table 1).
Preparation and analysis of samples for light microscopy, scanning electron microscopy, and histochemical localization of β-glucuronidase (GUS) activity in whole-mount tissue was done essentially as described [ 20, 80].
The applied procedures were induction of MS by fructose in drinking water; experimental protocol of walking and running; weighing of body mass and VAT; sacrifice of animals with blood collection and removal of organs and processing of samples for light microscopy using the analysis of volume densities (Vv) of the studied structures.
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Tissue samples for light-microscopic investigations were dehydrated with propan-2-ol, cleared with toluene and embedded in paraffin wax.
Sampling for light microscopy included a mean of 20.2 glomeruli (range 5 44), and a mean of 3.7 (18.3%) glomeruli were sclerotic.
> -wrap-foot> Tisamplingpling for light microscopy included a mean of 28 glomeruli (range: 21 42 glomeruli).
Among the 17 glomeruli sampled for light microscopy, three were globally sclerotic, and one was segmental sclerotic.
The nonlinear absorption in the sample for light at 1028 nm is dominated by carotenoids in pigment protein complexes [ 10].
The tissue samples used for light microscopy and immunoperoxidase staining were fixed in formalin and embedded in paraffin.
Figure 3(c) shows the stereological results obtained with electron microscopy analysis on the same samples used for light microscopy analysis.
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