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N.B. assisted with samples for initial MIBs/MS profiling.
Due to fixed samples for initial operation, the number of candidate samples remains constantly.
All experiments were conducted at 298 K. Samples for initial ligand screening used a concentration of 50 μM NHERF1 PDZ1 140140) and 2 mM ligand, in a buffer consisting of 25 mM sodium phosphate, 50 mM NaCl, 0.1 mM TCEP, 0.02% (w/v) NaN3, 1% (v/v) DMSO- d6, and 5% (v/v) D2O (pH 6.8).
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Cancer tissues sampled for initial pathological assessment have been used as pre-treatment specimens, while the surgically resected cancer tissues after chemotherapy regimen were utilized for post-treatment analysis.
16 Larvae were harvested at 5 dpf (∼30 per sample) for initial assessment of mitochondrial function and at 3 dpf to determine the effect of TigarB knockdown on mitochondrial function (see below).
as well as the lower numbers of samples used for initial evaluation but also to geographical patterns of marker occurrence in nontarget samples.
For smaller number of samples or for initial confirmation of variation, techniques such as HRM may be more appropriate as utilized in this case.
It is obvious that, for a fixed neural network structure, using more samples for the initial training would result in a smaller initial field estimation error.
J.Y. collected the total RNA for the RNA-seq analysis, and the genomic DNA samples for the initial de novo genome assembly.
Three SMKs are used as the samples for the initial test, while the rest is for the model test.
Table S2: Dilution of serum and urine samples for the initial measurement of CHI3L1 by ELISA.
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