It was found that 96-well filter plates provided contamination-free, rapid processing of samples for fiber-based enzyme assays using ToF-SIMS.
Three samples for each fiber dosages of 0, 4, 5 and 6 kg/m3 were tested after exposing at room temperature (27 °C), 150, 200 and 300 °C respectively.
Boll samples were ginned on a 10-saw laboratory gin, and fiber samples were used for fiber property analyses.
Samples were analyzed for fiber type, muscular atrophy and hypertrophy, fatty proliferation, fibrosis, presence of hyaline fibers, mitochondrial proliferation, muscular necrosis, nuclear centralization, presence of central core myopathy and inflammation (Table 1).> The muscle fiber type I was prevalent in the biopsies.
A preliminary study with the use of gastric juice showed that gastric juice is a less sensitive sample for evaluating fiber concentration in lung.
Samples were also evaluated for proximate composition (moisture, fat and protein) and a trained sensory panel evaluated samples for juiciness, muscle fiber and overall tenderness, connective tissue, and off-flavor [16].
The experimental design involved pairwise hybridizations between the two lines (TM-1 and im mutant), with two biological replicates and a dye swap, for fiber samples at 13, 16, 19, 22 and 25 DPA.
as can the almost systematic presence of a residual percentage of LAF fibers in SAF samples, the analytical methods for fiber analysis, the fiber type and the type of exposure and the metric (on a per weight basis the number of short fibers is greater than that of long fibers).
The strengths of this study include the rigorous statistical procedure used to perform the resampling analysis, the large sample of fibers for each rat with which it was performed, and use of a common laboratory animal model in muscle research.
Pigment coated paper fiber samples for SEM-EDS analysis were mounted on aluminum stubs using a carbon adhesive tab and coated with a 10 nm carbon layer.
