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Each patient provided two sputum samples for direct Ziehl-Neelsen light microscopy and Lowenstein-Jensen mycobacterial culture, after a laboratory technician provided standardized instructions on proper sputum submission.
A total of 8 weeks of sampling (including 5 samples/week) gave 40 samples for direct plating.
Examination of >3 stool samples for direct identification of Strongyloides larval forms has a sensitivity of 60 70%, and this may even be higher among immunocompromised patients.
The participants provided two spot sputum samples for direct fluorescence microscopy (FM) and Ziehl-Neelsen (ZN) microscopy; mycobacterial growth indicator tube (MGIT) and Lowenstein-Jensen (LJ) sputum cultures.
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The use of horizontal sampling for direct ΔG estimation led to systematically high errors.
All TB suspects with unknown HIV status were counselled and tested for HIV on the first day together with collection of a sputum sample for direct microscopic examination.
For TB diagnosis, patients provided a randomly-timed sputum sample for direct smear microscopy at the time of enrollment.
For malaria diagnosis, many NATs consist of a separate amplification and detection step because there is insufficient Plasmodium nucleic acid in a peripheral blood sample for direct detection by using current detection technologies.
The Twin-SHIME system, involving two models that run in parallel, was inoculated with the same fecal sample for direct comparison of the effect of the two polyphenol types.
With the revision of diagnosis and treatment guidelines, facilities are now able to obtain sodium stibogluconate by providing Pharmaciens sans Frontières with a positive rapid diagnostic test result, but they are encouraged to also take a blood sample for direct agglutination testing, as this is still considered more reliable (8 ).
The primers were useful for the analyses of poultry meat samples both for direct plating onto mCCDA, and for DNA extracted directly from the cells grown for 48 h in Preston enrichment broth.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com