Finally, the proposed method was successfully applied to 50 postmortem urine samples for determination of the opioids.
Between meals, participants provided exhaled breath samples for determination of gastric emptying; venous blood and appetite sensations.
Cardiovascular risk factors and DNA samples for determination of HO-1 GT repeats were obtained at recruitment.
Water samples for determination of selected physico-chemical parameters were collected at the initial, 7th, 30th and 40th day of the experiment.
Blood samples for determination of counts in the blood were collected at 0, 15, 30, 60, 120, 150 and 180 min.
During the first 48 h after admission to ICU, blood samples for determination of ACTH cortisol and prolactin were collected at 08 00 h.
Resting blood samples (for determination of glucose, insulin, and leptin concentrations), body condition scores, feed information, and health history were collected from 366 privately owned horses.
Radiochemical preparation of samples for determination of 14CO2, combustible 14C forms, and 85Kr was performed after the radioactive decay of radionuclides with short half-life.
The first 600 samples were employed for network training, the next 100 samples for the determination of the optimal weighted factor ω, and the last 100 samples for determination of the threshold of fault detection.
Samples for determination of glucose and insulin samples were placed into 1.7 ml chilled tubes coated with lithium fluoride and heparin containing 50 µl EDTA.
Blood samples for determination of glucose and hormones were obtained at the same time of day for all experiments (13.00 h) and under similar fasting conditions (4 h fasting).
