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Then MG-63 cells were seeded on samples for cell proliferation and ALP activity tests.
Samples for cell density and HPLC analysis were withdrawn from the cultures after 24, 48, 72 and 96 hours.
The topmost leaves of rice plants of each genotype grown under the control and chilling conditions were harvested at the same five time points as samples for cell membrane injury.
OD620nm was followed overtime (Ultrospec 2100 Pro spectrophotometer, Amersham Biosciences, Corp., USA) and samples for cell dry weight (CDW), extracellular metabolites and PHB analysis were collected in technical duplicate.
Samples for cell counts (1 mL for HC treatments, 10 mL for LC treatments) by fluorescent microscopy were also removed at the beginning and end of the experiment, and immediately preserved with 2% formaldehyde and refrigerated until processing.
The samples for cell cultures were coded and processed in the laboratory in an anonymous manner.
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Cell-surface markers were analyzed on ED presentation and on the third and seventh day of hospitalization in a subset of 523 subjects enrolled in hospitals located within 60 miles of the University of Pittsburgh because samples for cell-surface markers require analysis within 48 hours.
By analyzing the results of these tests, optimum sample for cell culture was selected.
The first single from Coldplay's next album is called, fittingly, "Speed of Sound," and it now is available in a 30-second sampling for cell phones, two months before the album shows up in stores.
The sample for cell imaging was obtained by fixing the stained cells using 4% paraformaldehyde on a 35-mm tissue culture dish (World Precision Instruments) and acquired the fluorescence images using LEICA TCS SP2 laser scanning confocal microscope with a 40×objective.
Additionally, relying on sampling for cell density and other measurements limits measurement frequency considerably.
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