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The samples for brain tissue distribution analysis were collected at the time of euthanization, with the whole brain being removed and thoroughly rinsed with cold phosphate-buffered saline prior to freezing on dry ice.
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Level of pitchfork-raising: Low, because this was obviously conceived by a PR agent with free samples for brains.
Equations from SAAM II compartment model used to derive TIAC in each source tissue (or sample) for brain, lungs, heart wall, kidneys, and liver are also provided.
Fish were collected post-passage, examined for visible external injury (eye hemorrhage, eye loss, descaling, and abrasions) and sampled for brain tissue following the conclusion of the standard 48-hour holding time for direct mortality (i.e., balloon-tag) observations.
Sample size for brain regions (varied due to availability and sample quality) included 8 posterior hypothalami, 6 anterior hypothalami, 7 diagonal bands and 4 LCs.
FACS isolation of EGFP-positive astrocytes was done using a FACS Aria II (Becton Dickinson) and gates were set according to control samples for each brain region from wild-type mice.
Since the original report of this phenomenon [ 30], based on the analyses of a few samples for each brain, the co-occurrence of PrPSc type 1 and 2 in a percentage varying between 12 and 44% of sCJD cases analyzed has been confirmed by a number of subsequent studies [ 13, 18, 20, 37, 40, 43].
The spatial sampling needed for brain magnetic fields was analyzed for SQUID-based sensor arrays in [ 31].
Samples of brain for the pathology based surveillance were collected from malformed bovine and ovine foetuses submitted for post mortem examination.
Normal brain tissue used as a template for microarray was obtained from samples of brain surgery for non-neoplastic disease.
LIDIVET receives samples of brain tissue for rabies diagnosis from suspected cases in animals and humans.
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