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Apart from the radionuclide calibrator and the SPECT/CT camera, the NaI(Tl) well counter is widely used in clinical setting in particular to quantify the activity in blood samples for bone marrow dosimetry in molecular radiotherapy [19, 20].
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After sacrifice and sampling for bone culture, tibiae were fixated in 4% formaldehyde/PBS for 4 weeks and embedded in polymethylmethacrylate (PMMA) (Technovit 9100, Hereaus-Kulzer, Germany).
The antibiotic treatment for osteomyelitis should ideally be initiated after obtaining sufficient samples from bone for microbiological assay.
The sample size for bone volume and bone mass density measurements at Day 70 for SFCS controls was n = 1 and was not used for comparisons.
Two serum samples were collected for bone markers, 1 week apart, prior to initiation of therapy; baseline levels were the average of these two values.
Fasting urine samples were analysed for bone resorption markers; CTx-I (RatLaps; Nordic Bioscience Diagnostics A/S), helical peptide (Metra, Quidel Diagnostics) and free and total PYD and DPD (Metra, Quidel Diagnostics).
Samples for markers of bone turnover: cross-linked N-telopeptides of bone type I collagen (NTx) as a bone-resorption marker and osteocalcin, bone-specific alkaline phosphatase (BALP) and intact N-terminal propeptide of type I procollagen (PINP) as bone-formation markers were stored in the same manner.
A series of individuals excavated from the cemetery in 1975-77 have previously been sampled for compact bone tissue which were analysed in triplicate for Hg by Cold Vapour Atomic Absorption Spectroscopy (CV-AAS) [3].
The activity profiles show that the expression modules in the first cluster, V$E2F4DP1_01 and V$NFY_01, are upregulated in a sample subgroup enriched for bone marrow, lymphoma and leukemia cells.
Samples for the alveolar bone were obtained using a Master 3D CT X-ray tool (Vatech Korea, Seoul, Republic of Korea), with settings of 90.0 kVp and 30 mA.
This would also enable the reserve of valuable cord blood samples for use in bone marrow transplantation.
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