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Blood samples for biochemical analyses were obtained from the hearts.
Blood samples for biochemical analyses were drawn in the morning after an overnight fast.
After thawing the saliva samples for biochemical analysis, the fraction of free cortisol in saliva was determined using a time-resolved immunoassay with fluorometric detection.
On day 7, overnight-fasted rats were anaesthetized and then euthanized by CO2 inhalation, 3 h after the last treatment with SSR240612, to collect tissues and blood samples for biochemical and molecular studies.
Blood samples for biochemical analyses were drawn at weeks 0 and 33 after a 12 hour overnight fast from an antecubital vein while a subject was in a sitting position.
Samples for biochemical and hematological analysis (2 mL of blood), including reticulocyte analysis, were taken on Day 0, Day 3 and Day 7 from all patients and on Day 14 if any value was abnormal on Day 7. Samples were analyzed locally at each investigational center.
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Sampling for biochemical markers was standardised by obtaining blood at a fixed hour after an over-night fast.
60 days after end of treatment, 24-hour urine was collected from rats, and subsequently animals were euthanized under anesthesia (zolazepam + tiletamine 1 1, 20 mg/kg) via instantaneous decapitation with blood sampling for biochemical and immunological tests.
At 21 day, mice were euthanized and tissues were sampled for biochemical and histological analysis.
A blood sample for biochemical measurements and DNA extraction was taken from each individual.
Then all participants underwent blood sampling for biochemical assays and flow cytometry.
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