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After 60 min of collecting samples for baseline, the rats were injected either with the A2AR antagonists KW-6002 or SCH-442416.
Venous blood samples for baseline cortisol levels were drawn at approximately 0800 0900 h, followed by 25 mg oral cortisone acetate (Cortal® 25 mg, N.V. Organon Oss Holland).
Blood samples for baseline determinations were collected from each patient after inclusion and just prior to initiating paricalcitol treatment.
Quantitative real-time PCR (qRT-PCR was used to analyse expression of seven of the differentially expressed genes in a subset of 73 samples (for baseline characteristics of this subset, see online supplementary table S4).
After collection of three 30-min interval microdialysis samples (for baseline glucose measurements), mice were given an intraperitoneal injection of 250 mg glucose in PBS/kg body weight during continuous microdialysis sampling.
To obtain standard samples for baseline analysis, 2 g of non-treated heartwood and sapwood samples from each wood species were mixed with approximately 0.45 g of diatomaceous earth, and then transferred to an 11 mL cell; the headspace in the cell was then filled with sand.
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Soil amendments were prepared and sampled for baseline analyses.
This gene-by-gene response also highlights the need to use a matched sample for baseline measurements of dosage compensation.
A research assistant arranged to meet the participants the same day the 24 h collection was completed, and a blood sample for baseline laboratory measures was obtained.
After 2 h of controlled blood sampling for baseline LH pulses, the animals were exposed to one of the three stressors.
Before the doubly labeled water was administered, subjects underwent a general health check, anthropometric measurements and were asked to provide a predose urine sample for baseline isotopic measurements.
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