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Junger has the elements of a classic mystery, though this one will never be solved, as everyone involved is long dead, and no DNA samples exist to prove or disprove anything.
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Few fine-grained samples exist due to the prevalence of Strombolian and lava-fountaining activity in recent years, which tend to produce coarser ash (including the large, December 2015 paroxysmal eruption; Corsaro et al. 2017).
With appropriate sampling, methods exist to estimate the within-host effective population size, as well as the mutation rate (e.g., Wang 2001; Minin et al. 2008).
Where two samples existed at one location (e.g. two different depth), the maximum uranium concentration was used to be more protective of human health.
Specific data forms exist to track samples and to record infectious events, death and drop-out.
In summary, a variety of methods exist to collect samples to assess potential exposure to pesticides from environmental media.
Several methods exist to calculate sample size for the difference of proportions (risk difference).
Many national sampling and hospital surveillance systems exist to monitor antimicrobial resistance patterns in bacteria (1 – 4 ).
This design has become a standard method of assessing health and nutrition status in emergency situations, where populations are large and no sampling frame exists to permit simple or systematic random sampling.
Few historical environmental samples exist, and evidence that such strains made the jump to humans is even harder to come by.
For the biomonitoring programs a system ensuring optimal use of samples is needed – it can be argued that it is not ethically justifiable to collect new samples if suitable samples exist in a biobank.
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