This allowed for detection of residual host cell DNA of much lower concentrations and, for some samples, eliminated the need for DNA extraction.
Furthermore, the RT-LAMP procedure was modified for the direct detection of HIV-1 nucleic acid in plasma and blood samples, eliminating the need for an additional nucleic acid extraction step and reducing the overall procedure time to approximately 90 min.
The latter is of particular logistics importance during epidemics and drug discovery efforts, as it facilitates the simultaneous and cost-effective screening of multiple samples, eliminating the need of tedious visual examination of numerous cultures.
As an alternative, the data can be split, with weights derived in a training set and association testing undertaken in the remainder of samples, eliminating the need for computationally demanding permutations [ 24].
We believe that our work represents a first step toward bridging current imputation practice with the paradigm suggested by Kong et al. (2008), in which large population samples eliminate the need for complex models and reference panel selection, and investigators do not have to balance efficiency and accuracy.
The surface area was measured with the confidence ellipse including 90% of the CoP positions sampled eliminating the extreme points [25].
On the other hand, taking into account only genes differentially expressed on the 102 ARMS and ERMS Davicioni's samples (eliminating all the other RMS subtypes) the number of confirmed genes increases to 7348%8%, p = 0.01, 55 down and 23 up regulated) [Additional files 1 and 3].
Use of a single reference sample, processed using the same bench protocol as used for the non-reference samples, therefore eliminates the need to normalize for GC content and for sequence uniqueness within each block.
First, we showed that there was no expression of exFeLV in all samples, which eliminates the possibility of recombination between exFeLV and enFeLV.
