DNA was extracted from HBsAg positive samples by using the QIAGEN DNA Blood Mini kit (QIAGEN, Venlo, the Netherlands) according to the manufacturer's protocol.
For the Rt qPCR, cDNA was obtained from RNA samples by using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with oligo dT primers.
Move the populations of samples by, using the genetic transition kernel according to.
Genomic DNA of all the rice accessions was extracted from the leaf samples by using the CTAB DNA extraction method.
Low-dilution glass beads can be an adequate target to analyze trace element composition of rock samples by using the laser ablation ICP-MS.
Since the received signal samples are correlated in the time domain, we estimate the missing samples by using the expectation-maximization (EM) algorithm.
In the case where the true path delay is different from integer times of delay samples, by using the maxima-searching method, large estimation errors are resulted.
(iii) Move the populations of samples by, using the genetic transition kernel according to crossover, mutation or exchange steps as described below.
Briefly, total RNA was obtained from samples by using the Trizol reagent (Invitrogen).
Genomic DNA was extracted from whole blood samples by using the standard method.
MSP3-family gene fragments were PCR amplified from these DNA samples by using the primer pairs (SI, Table 1B).
