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We also managed to reduce heterogeneity in genetic background by including only ethnic Chinese Han individuals, and in disease phenotype (in our family samples) by excluding subjects with family history of hypothyroidism [13].
We are able to align the two samples by excluding the consecutive birth cohorts from the GSF sample which are not part of the NSS sample, and repeat this for all four years of the NSS sample (see Figure 2).
We chose to evaluate gene expression profiles in the low passage cultured cells, which we surmised would provide a more homogeneous population than fresh tumor samples by excluding non-malignant stromal components and inflammatory cells, mitigate genes associated with phenotypic endothelial commitment, and diminish the influence of differential proliferative signatures.
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We restrict our sample by excluding those aged below 25, who are likely to be still in school.
Total RNA libraries were prepared from aliquots of the biotin-UTP-treated NRO sample by excluding the steps of nascent RNA selection on streptavidin beads.
We will then trim the sample by excluding patients with a propensity score corresponding with the 2.5th centile or lower in the varenicline, respectively bupropion group and by excluding patients with a propensity score corresponding with the 97.5th centile or higher in the NRT Rx group.
We further limited the sample by excluding patients who had a Medicare hospice claim during the 12 months prior to the index admission date, and to ensure that we could measure Part D outpatient claims accurately, we required patients to survive 30 days post institutional discharge date without a readmission to a hospital, hospice, or skilled nursing facility [ 5].
Although we attempted to correct for the bias in taxa sampling by excluding several species of Drosophila and mammals from our data set, a large overrepresentation of sequences from Insecta and Vertebrata remain, limiting the interpretation of our results for other animal groups.
The sample formed by excluding these two subpopulations from the greater population of patients with shoulder pain or disability is no longer representative of typical clinical practice.
This dataset was further reduced to those showing a >2-fold deviation from the mean of all five conditions in at least one sample, and by excluding all genes encoding VSG/VRs and ESAG/GRESAGs (which were analyzed separately, see below).
This survey collected 4610 valid questionnaires, with an effective sample size of 2098 by excluding the part of the sample with incomplete information on variables related to this study.
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