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The feature data of each class are obtained from the samples by calculating the average feature data of all samples.
Natural moisture content was determined in both soil samples by calculating the ratio of the weight of water to the weight of solids in 500 g of each sample according to ASTM D2216-90.
For the tree based on mRNA expression, we created pooled samples by calculating the mean expression value for each gene based on expression levels in two individuals of the same species (Table S2).
Because RNA isolated from intracellular parasites contained a large portion of host cell RNA, the levels of parasite 18S rRNA were first used as baselines to normalize those of CpT6PS-TPase transcripts in all samples by calculating the ΔCT (i.e., CT[CpT6P-TPase] - CT[Cp18S]) for calculating the relative levels of CpT6PS-TPase transcripts with 2−ΔCT.
A script automatically visualizes the results as cluster diagrams for each SNP and genotypes the samples by calculating allelic fractions (AFs).
Rapaport and co-workers found gene expression patterns of neighboring genes in the network yielding good classification of the profiled samples by calculating Fourier transformations and rejecting high frequency signals [ 18].
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It was further heated at 750°C for 4 h to find the percentage of carbon in the sample by calculating the reduction in weight.
Parceled indicators were computed for the present sample by calculating the mean response for the shortened list of items representing a particular subscale.
We examined the characteristics of the study sample by calculating the mean values of continuous variables and frequencies of categorical variables.
We determined the crossing points of the amplification curves for the variable and the common DNA tag region for each sample by calculating the second derivative maximum (LCS480 1.5.0.39 software).
We investigated differences between multiple characteristics (age of onset, gender, LC subtype and MDM2 SNP390 genotype) of both cases-samples by calculating a propensity score, which is defined as the conditional probability of assignment to a particular case-sample given a vector of observed covariates [ 14].
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