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Assays employed were commercially available enzyme-linked immunosorbent assay conducted following manufacturer instructions, with all samples batch analysed upon study completion and samples from each participant assayed on the same plate.
With these samples, batch experiments were carried out according to the German technical guideline VDI 4630 [19].
We prepared 4 sets of 12 samples from a batch of 48 samples (Batch #3) and performed the genotype call comparison for each set of 12 samples.
This class information was used to train an O2PLS-DA classifier on the same samples (batch 1&2 samples) based on their batch 1 and 2 metabolomics measured data.
The remaining 731 samples (Batch 2) were analysed by liquid chromatography/mass spectrometry, in which the dried extracts were redissolved in 40% aqueous methanol and were analysed as above.
There were a total of 80 homogenate samples (batch of 10 cockroaches pulled as one); 40 were gut homogenates and the other 40 were external homogenates, the samples were collected sequentially; the first sample was collected in February and the last in July.
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Asexual parasites were tested in 3 separate experiments, with plasma samples batched by year of collection.
Within days, a sample batch was on its way to Gehry's office.
Variations in sample batch, number of spectral scans, and the interactions between sample batch*soneration time, no.
A mobile regolith origin under, at least seasonal, periglacial conditions is indicated where clay ≤ 0.5*silt + 8 across a sample batch.
Solvent blanks were run with each sample batch.
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