Our approach is consistent with previous studies that used gastric mucin to examine the viscoelastic properties and biological applications of mucins [51], [52], [53], [54], [55], [56], since there is currently no commercial mucin sample available that can be preserved in the native state.
Briefly, from the archived serum samples collected between 1976 and 1985 and stored at –20°C, we preferentially selected for analysis the first sample available that was collected between 1976 and 1981 and was of adequate volume (> 0.5 mL) to measure TCDD.
Forty-eight serotypes, including CBV5, were not detected in Kerala, probably because of the relatively small number of samples available from that state or noncirculation of these serotypes.
Visit 4 measurements were excluded from the average because of a relatively small proportion of cases with samples available from that time point.
For the present analysis, 50 samples were randomly chosen from 1997 and 2011 (of 160 and 197 samples available from that year, respectively) to provide two temporally distinct contemporary population samples for comparison with the historical data.
For both subtypes, the total number of migration events associated with each node in the network is correlated with the number of samples available for that node (all p values were <10−5; Kendall and Spearman tests).
Samples were available that had been analyzed in a previous study of two Nacella species from the South American Magellan region (N. magellanica, N. deaurata), analyzing the heat shock response (HSR) on a shore level and a North south geographical climate gradient [ 20].
This algorithm was easier to implement and work with, and had sample code available that was compatible with AVR.
Loss-of-heterozygosity (LOH) analysis revealed loss of wild-type allele in all tumour samples available, suggesting that AIP is likely to act as a tumour suppressor gene.
