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The two platforms gave similar results for pooled tissue samples, as summarized in Figure 6 and tabulated in Additional file 4 Table S1.
Among those 94 GBM samples, as summarized for the patient characteristics in Table 1, 67 cases were evaluated by quantitative RT-PCR analysis for MRP3 mRNA transcript expression and 46 cases were evaluated by IHC analysis for MRP3 protein expression.
We used individual small RNA data sets to predict miRNA genes for C. elegans, P. pacificus, and S. ratti in developmentally arrested (data sets 4, 8, and 10) and mixed-stage (data sets 1, 2, 5, 6, and 9) samples as summarized in supplementary figure S1 A, Supplementary Material online.
Commensurate with RNA-Seq studies [ 21] and microarray studies [ 22] in developing whole Arabidopsis root samples, as summarized in [ 3], our nanoCAGE-XL libraries covered ~20,000 of the ~27,000 total protein coding genes in the Arabidopsis genome; over 16,000 of these genes have a high confidence TSS peak.
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The microalgal biomass used in this study showed a relatively high amount of polysaccharides since a strong absorption peak was recorded around 1100 cm−1 to 1000 cm−1 in the powdered microalgal sample as summarized in Figure 1.
In comparison with numerical results of these community-based studies (mostly with inclusion of dementia cases) [ 5], the effect of increased age and cognitive impairment was lower for our dementia-free sample as summarized for community-based studies (HRs for age: 1.09 vs. 1.06-7.72, and cognitive impairment: 1.19 vs. 1.59-1.67).
Detection limits are usually expressed as a concentration, i.e., they are based on the average weight of the sample analyzed, as summarized in Table 6.
There are three main factors that must be considered in the calculation of an appropriate sample size, as summarized in Table 1.
We have also identified single gene discriminators for each grade group against the rest of the samples, including the control, as summarized in Table 1.
Then, the resected specimen should be sampled and examined properly, as summarized in Steps 1, 2, and 3, warranting not leaving out any prognostically relevant samples, particularly those containing the relationship of the lateral margins with the primary tumour and mesorectal nodules.
We performed a paired t-test, comparing the mean of the features from the Positive936 data set with the mean from the Dishuffle936 data set, and computed the P-value estimating the probability that these samples have the same means, as summarized in Supplementary Table S1.
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CEO of Professional Science Editing for Scientists @ prosciediting.com