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Organic acids, including citrate, were extracted from leaf samples as previously described (Phillips and Jennings 1976), with some modifications.
Bisulfite sequencing was performed in 9 samples as previously described [20].
The SVCV-C1 strain was isolated from tissue and water samples as previously described.
IgG anti-HEV avidity test was conducted on some serum samples as previously described [18], [31].
For electron microscopy, we fixed the dissected PSGs and processed the samples as previously reported [55].
Subsequently, we performed gene dosage analysis in two samples as previously detailed.
Total RNA was extracted from tissue samples as previously described [17].
DNA was extracted from cryopreserved PBMC samples as previously described [63], [64].
DNA was extracted from all available filter paper blood-spots corresponding to slide-positive samples as previously described [25].
The intracellular cytokine flow cytometry (CFC) assay was performed using frozen PBMC samples as previously described [53].
Confocal microscopy was performed on biofilm samples as previously described using LIVE/DEAD® BacLight™ Stain [20], [21].
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