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Soil bulk density and moisture contents at each sampling depth were determined on the undisturbed core samples, as outlined in Blake and Hartge [37].
Next to the determination of biofilm mass and planktonic growth, viable cell counts and fluorescence microscopy were performed on the samples as outlined in the Materials/Methods section.
The presence of the human G93A transgene was confirmed using PCR amplification of DNA extracted from tail samples as outlined by Jackson Laboratories.
We optimized an isolation system designed specifically to deplete only the CD25hi cells from our samples as outlined in methods (Figure 4a).
Alternatively, a meta-analysis of existing CFS data sets would provide a valuable extension with the potential to identify gene expression signatures for formal assessment as disease classifiers in new samples, as outlined here.
Thus, mixture experiments with these samples as outlined above created a picture that resembles LH.
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Groundwater samples were collected as outlined by Fitfield and Haines [29].
Peak heights were analysed for the eight probes for each DNA sample, as outlined in White et al [12].
The adapters were prepared and combined with 100 ng of DNA sample as outlined in Elshire et al. [ 3].
Material from the samples was prepared in sterile physiologic saline and injected into 4 mice per sample, as outlined by Ajello and Weeks (9 ) for environmental isolation of Histoplasma capsulatum.
Uninfected (control) and bacterial-infected leaves excised at phases I and II of HR, were fed with C-Pro (30 min), transferred to water for different time periods (up to 60 min), and sampled as outlined in Figure 2B.
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