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The DUX4 5' BSS analysis was tested on the same eight genomic DNA samples as above.
From those who agreed, the same data and samples as above were obtained.
The same samples as above were sequenced using commercial kits and protocols provided by Life Technologies, Carlsbad, USA.
We clustered the samples as above and compared these clusters to the class labels predicted by the decision tree model with Cohen's kappa statistic [ 24, 25].
Using these randomly chosen 70 samples instead of 501 samples as above, we computed the PMP-sim for all 27578 loci again and observed high PMP-sim values for the CTA and CTX genes.
The same blood samples as above were analyzed by flow cytometry and indirect ELISA.
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Then, randomly generated observations from that normal distribution were sampled as above.
In addition, ΔCtendo was calculated by using endogenous control Ct values for the same calibrator sample as above, and subtracting subsequent endo Ct values from that calibrator [55].
In subsequent trials, fish were sampled as above, but at seven and 14 dpe (Trial 2) and at 28 and 43 dpe (Trial 3).
Peaks of two samples identified as above were merged as candidate differentially methylated regions (DMR).
The same RNA samples as described above were now subjected to the vRNA removal protocol.
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