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The enrichment-ELISA can be made quantifiable by a most probable number approach in which replicates of serially diluted test samples are verified.
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DNA samples were verified by multiplex PCR assay (Supplementary Table S6) to exclude cross-contamination.
The HPV 16 samples were verified by a standard PCR test.
Genomic DNA samples were purified from cultured fibroblasts and iPSC lines using PureLink Genomic DNA Mini kit (Thermo Fisher Scientific), which was previously used to quantitate mitochondrial DNA copy numbers.57 The quantity and quality of DNA samples were verified by 1.0% agarose gel electrophoresis.
The quality of the extracted samples was verified by polymerase chain reaction (PCR) and gel-electrophoresis after the extractions.
The crystallinity and purity of the samples were verified by XRD (Cu-Kαradiation) and thermo gravimetric measurements (20 °C/min).
The SH and DH structures of the samples were verified by high-resolution transmission electron microscopy (HRTEM).
An extended stability of the DNPH-glucose derivative in spiked plasma samples was verified via the employed HPLC-UV method.
The pigment samples were verified analytically as described in [53] and the paint samples were prepared in animal glue and painted on paper [52].
All of our positive samples were verified with sequencing.
Sequences from several samples were verified by extraction and amplification at the Griffith University Ancient DNA Facility, Nathan, Australia.
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