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The correlation analysis indicates that river water samples are hard.
In post monsoon 44% of the samples are hard water and 36 are very hard water.
It is possible to evaluate skin mechanical properties based on human or animal experimentation, yet in addition to possible ethical issues, these samples are hard to obtain, expensive and give rise to highly variable results.
According to classification by (Swayer and McCarty 1967) the TH of the groundwater shows that 55% of the samples are hard water and 22% are very hard water in pre-monsoon.
Secondly, negative samples are hard or even impossible to select as there are no verified negative miRNA-disease associations.
As EMT processes in human samples are hard to follow and because of the difficulty of detecting CTCs in humans [ 8], animal models could help to evaluate and dissect the functional implications of EMT specific processes in vivo.
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Among the water samples collected, 2 and 1% of water samples are harder in pre- and post-monsoon.
The reason why we had to take this step was that NGS samples are harder (and much more expensive) to obtain than microarray data, and the number of samples was small, which may have caused overfitting in the parameter estimation process of our model.
Pure samples were hard to obtain; chemists didn't know how to isolate their components, and medical reports were contradictory.
Chasing samples is hard work, said Sabrina Locatelli, a State University of New York primatologist who works in Africa and collaborates with Dr. Hahn.
Most of the samples were hard (48.7 %) with TH ranging between 150 and 300 mg/l.
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