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The samples are fixed with 2.5% glutaraldehyde in Dulbecco's phosphate buffered saline for 20 min at room temperature, washed thrice with deionized water, and then air-dried.
The samples are fixed on the Faraday cup and irradiated with electrons at a pressure ∼10−7 mbar.
Take the three steps for engineering experiments: first of all, the experimental samples are fixed on the experimental platform placed on the mountain.
Chen and Hsieh (2007) indicated that traditional T 2 chart gives a better performance if both sample size and control limits are variable (VSSC), and the waiting time between successive samples are fixed.
The initial and final dried vermicomposted samples are fixed on a circular metallic sample holder by using double-sided adhesive tape and then the samples are coated with gold using sputter coater for clear visibility of picture, followed by micrographs of surface texture at different magnifications of SEM (Bhat et al. 2015a, b).
For most FRET measurements via fluorescence microscopy samples are fixed and mounted on cover slides.
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Cultured samples were fixed in 4% paraformaldehyde, frozen and sectioned prior to histological evaluation.
For flow-cytometric analysis of mouse lung and BALF, samples were fixed using 4%PFA.
Skin samples were fixed in 10% formalin, embedded in paraffin, and cut into 7-um sections.
Samples were fixed with 3% paraformaldehyde plus 0.5% glutaraldehyde as described above.
Samples were fixed with 4% paraformaldehyde at 37 °C for 20 min.
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