The samples and substrate solution were incubated at 25°C, 40°C, 60°C, or 80°C for approximately 5 min. The lysozyme activity of the samples was then quickly measured by the turbidimetric method described above, using triplicate samples.
The interface layer between the polymer and the substrate is a dead layer, which shows no mobility due to interaction force between sample and substrate [4].
While some variation is a result of sampling and substrate heterogeneities, a significant portion of the variation is a result of baseline artifacts introduced by the baseline subtraction algorithm.
The confinement effect prevents further sample and substrate dilution into the entire cell culture plate.
When sample and substrate have closer value of n, this systematic variation decreases (e.g., ZnS) or becomes zero (CaF2).
Selected coated samples and bare substrates were exposed to an atmosphere of CH4 + H2 + residual oxygen at 800 °C for up to 20 h via thermogravimetric analysis (TGA).
Distilled water was used to prepare all the solutions in our experiments, and ethanol and distilled water were used to rinse the samples and glass substrates.
Table 1 Results of XPS investigations, with surface concentrations given in atomic % (at%), performed for polished AM50 samples and AM50 substrates in contact for distinct periods with aqueous (aq).
The tribological behaviors of Mo Cr duplex-alloyed samples and bare substrate were investigated using a ball-on-disk tribometer under the conditions of lubrication-free at room temperature.
The macroscopic wettability state of the samples and glass substrate was estimated by measuring the static contact angles of sessile water drops placed on a sample surface.
