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Samples and standard curves were always processed in duplicates.
The samples and standard solution of Trolox were daily prepared.
YTK and SA carried out the HPLC analysis of the samples and standard.
Balance (Adventurer, OHAUS, China) was used to weigh the samples and standard solutions.
Means of four independent samples and standard error values (n = 4) are indicated.
Means of three to four independent samples and standard error values (n = 3 to 4) are indicated.
The TTB measures were absolute minimum TTB, mean of the minimum TTB samples, and standard deviation of the minimum TTB samples.
Suitably cut circular filter paper pieces containing 100, 50, 25, 12.5, 6.25 mg/mL antibiotic solutions test samples and standard were introduced into the media.
Lanthanum chloride (0.5%, w/v) was added to both samples and standard solutions to avoid chemical interferences, as referred by Kawashima and Valente-Soares [25].
All the measures were taken as the average of minimum three biological and analytical replicates from different test samples, and standard deviations were calculated.
The intensity of the IR fluorescence of the samples and standard probe was detected at 800 nm with the Odyssey scanner.
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