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Table 1 The preparation conditions for the samples and quantitative ratio between oxygen and cobalt Deposit V e (kV) I e (nA) O/Co I(L2)/I(L3) 1 30 0.044 0.85 0.27 2 30 2.400 0.04 0.30 Summary of growth parameters, beam energy (V e) and current (I e), EELS quantification ratio between oxygen and cobalt and the average L2/L3 intensity ratios in Co L2,3 edge (see text for details).
Decoding of samples and quantitative mapping to the input amplicon sequences concludes the experimental workflow.
Conventional metallographic investigations can be performed on the quenched samples, and quantitative parameters, such as phase area fraction and parameters of the dendritic structure can be determined.
Using the same RNA samples and quantitative real time PCR, we were able to corroborate induction of miR393, a miRNA previously identified as nitrate responsive [ 37].
Once we had selected the potential markers, we checked their expression levels initially in lung cancer tissue samples using microarrays and then further validated them using patient's blood samples and quantitative RT-PCR (qPCR).
GC-MS data was processed using AMDIS [ 68] for peak detection from multiple randomly selected samples and quantitative peak area data extracted using default settings in MET-IDEA [ 69].
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For every sample and quantitative target extracted intensities from corresponding integration bin were summed.
An ArF excimer laser system (λ = 193 nm) with imaging optics for controlled UV ablation and simultaneous petrographic viewing was designed specifically for representative sampling and quantitative multi-element analysis of microscopic fluid, melt and mineral inclusions beneath the sample surface.
To facilitate individual sampling and quantitative collection of all voided faeces without handling the animal, the method described by Touma and colleagues was used [31], [32].
Image acquisition, dendrite sampling and quantitative analyses were performed as previously described (Nwabuisi-Heath et al., 2012).
This was still the case when Europe-defined PN2 cases which are based on the criteria "non-protective sample and quantitative culture" were considered as clinically defined PN.
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