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Density of immunoparticles were averaged from different samples and presented as mean ± S.E.M. Group differences were compared by unpaired Student's t test (two-sided); p<0.05.
All data were obtained from triplicate samples and presented as fold changes of mRNA levels, compared with the indicated controls in the graph.
The molar amounts of the GPCR genes and Rp49 were calculated, and the transcript levels of the GPCR genes were normalized with Rp49 levels in the same samples and presented as arbitrary units.
The colony number was calculated and plotted as the mean for triplicate samples and presented as percentages relative to the control.
Viability was calculated from relative dye intensity of the mean for duplicate samples and presented as percentages relative to vehicle samples.
Another publication assessed the validity of using self-collected cervical samples, and presented a basic analysis of correlates of HPV infection in the urban sample only (see Tracy, JK et al. 2011 [ 9]).
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The data originate from complex samples and present a hierarchical structure.
The pooling strategy necessary to obtain sufficient starting material for the fetal muscle samples may have reduced variation present between individual samples and present a less variable overall miR profile.
All data were normalized to elongation factor 1 alpha (GenBank Accession: HQ687762) expression from the same sample and presented as a ratio relative to ef1α.
The levels of target gene expression were calculated following normalization of endogenous control for each sample, and presented as relative units.
Data is normalized to a reference sample and presented as percent methylated reference (PMR).
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