The total amount of ammonia released was analyzed by volumetric analysis of both the reactor outlet gas sample and liquid reaction crude against strong 0.5 M HCl.
The MODS assay utilizes an inverted light microscope to visualize the characteristic cording growth of MTB through the underside of 24-well tissue culture plates previously inoculated with a decontaminated sputum sample and liquid culture media.
The theoretical transmission spectrum has been modeled using the transfer matrix method and includes the effect of the sample, transducers, and liquid coupling the transducers to the sample.
Improvements in fractionation, purification, and separation techniques in sample preparation and liquid chromatography (LC) and increased resolution and sensitivity of MS devices are required but remain challenging.
Refer to Additional file 8 for complete details on the proteomic sample preparation and liquid chromatography – mass spectrometry (LC-MS/MS) analysis.
LC, for example, pushes a sample and a liquid solvent through an adsorbent-filled column.
When saturating the slag in spinel by adding Fe2O3, a spinel layer forms at the interface between the refractory sample and the liquid slag.
In total 320 μL was loaded per sample and the liquid flow rate was 0.2 mL min-1.
