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The San Diego Zoo has its parallel Frozen Zoo, an archive of thousands of DNA samples and cell lines from a host of species.
Another specific and sensitive tool for diagnosing chikungunya infection is the one-step TaqMan RT-PCR assay that quantifies viral load in both clinical samples and cell culture.
Polymerase chain reaction assays have become widely used methods of confirming the presence of Mollicutes species in clinical samples and cell cultures.
We conducted analysis to identify genome-wide aUPD regions using data from 700 breast tumor samples and cell lines.
As shown in Figure 1a and 1c, the edited substrates were amplified successfully in all tissue samples and cell lines.
Interestingly, these two genes are overexpressed in the great majority of primary NB samples and cell lines.
As a matter of fact, miR-21 was the most significant downregulated miRNA in ovarian cancer when our data was averaged across all samples and cell lines.
ELISA testing of serum samples and cell culture supernatants was performed using an Erythropoietin Receptor DuoSet ELISA (DY307, R&D Systems, Minneapolis, MN).
Here, we report that AKT phosphorylation is elevated in both RMS tissue samples and cell lines.
RNA was prepared from ovarian samples and cell lines as described previously (Sellar et al, 2003).
All human tumor samples and cell lines were prepared in the same manner as above.
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