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Three years ago Dr. Rosalind Harding of Oxford University and others made a worldwide study of the MC1R gene by extracting it from blood samples and analyzing the sequence of DNA units in the gene.
Protocols for running real-time RT-PCR, normalizing between samples, and analyzing results have been previously described [9].
A proper test of the contending models of the architecture of the phenotype can only be undertaken by studying population based samples and analyzing measures that cover the full range of manifestations of the putative quantitative traits.
Allele distributions were determined by screening the panel of vervet DNA samples and analyzing fragment lengths.
The test set was blinded to personnel processing the samples and analyzing the data.
Using 50 pooled samples and analyzing 10,000 SNPs with a minor allele frequency of >1% and applying the best correction method for the corresponding type of comparison, the 90% quantile (median) of the pooling-specific absolute error of the RAS values for single sub-pools and the SNP-specific difference in allele frequency comparing two pools was 0.064 0.0266) and 0.056 0.0211), respectively.
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ACG and JP grew the samples and analyzed the data.
PHS grew the samples and analyzed the data.
BH, QY, BY, and XH helped prepare and characterize the samples and analyze the data.
JH, GS, and FQ prepared the samples and analyzed the data.
DNA was extracted from the samples and analyzed with a PCR-based identification assay.
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