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Further investigations concerning TP53 mutations and expression should be done in larger samples, also including early GC specimens.
We collected 92-matched samples also including samples from a third institute: 64 patients (tumoral and peritumoral paraffin-embedded tissues) from RENCI cohort, 16 patients (tumoral and peritumoral paraffin-embedded tissues) from SVH cohort, and 12 patients with tumoral and peritumoral frozen tissues from SAH in Rome.
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The tephra samples also include foraminifers and calcareous nannofossils.
The samples also included another estimated 64 genera that were not in the databases.
Biological samples also include blood collection, as described in the list of research activities eligible for expedited review.
A third cluster of 13 samples also included 4 tumors with an activated SHH signaling, one of them with a PTCH1 mutation as well.
Non amplified samples also included 5-cell (n = 4, 4 blastomeres), 6-cell (n = 2, 11 blastomeres), and 8-cell (n = 4, 30 blastomeres) embryos, as well as the blastocysts (n = 5) biopsied for ICM and TE isolation.
Serial sections, on our RCC samples, also included non-lesional areas, 5 cm distant from tumoral mass.
CNV results are based on Bickhart et al. [ 21] that use a Holstein, a Nelore, a Hereford and 3 Angus samples, also included in the FST analyses.
The HFP_G5C samples also included 0.2 mole fraction DTPG (ditetradecylphosphatidylglycerol) lipid, which reflects some negatively charged lipids in host cell membranes.
The experimental samples also included native TTR variants (0 M urea, 100% folded) and 100% unfolded TTR to serve as references.
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