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All the samples achieved an average coverage of 200× in the regions where SNPs were called.
All samples achieved an RNA integrity number (RIN) score greater than 9.5 [ 40].
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MAPP treated open hole samples achieved a reasonable improvement in tensile strength for all kind of laminate lay-up.
Eye movements were recorded during the procedure and participants who spent more time observing the samples achieved a higher percentage of correct responses in this task, whereas participants who spent less time observing the samples achieved a lower percentage of correct responses.
All replicate samples achieved a correlation coefficient of greater than 0.985 (data not shown).
The results of qPCR were analyzed in the same way: the binary coding for qPCR reactions was based on whether or not mouthwash-extracted DNA samples achieved a threshold level of amplification (the threshold level having been chosen to approximate the minimum amplification level sufficient for successful microsatellite genotyping).
All samples achieved a PCR duplicate rate of less than 10%, with a mean of 3.78%.
About 4.7% of the random samples achieved a prediction accuracy greater than 76%.
*Validation of the model based on 100 bootstrap samples achieved a mean c-index of 0.579 955% CI: 0.576 – 0.583), indicating good internal validation.
A follow-up study with additional samples achieved a genome-wide significant score for a region of 6q, indicating the frequent presence of genetic variation in this region that modifies HD pathogenesis prior to neurologic onset [ 54].
The multivariate model obtained with the RLS analysis was used to classify the available samples, achieving an accuracy of 91%.
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