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Briefly, a Thermo Finnigan TSQ Quantum Ultra tandem mass spectrometer equipped with an electrospray ionization (ESI) source (San Jose, CA, USA), a Finnigan surveyor LC pump, and an auto sampler were used for LC-MS/MS analysis.
Separate equipment, including a pump (Gast Oil-less Pressure/Vacuum Pump; Gast Manufacturing, Inc., Benton Harbor, MI) and an Andersen two-stage sampler were used for each location on the site.
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In addition, 24 normal breast samples were used for comparison.
Milk samples were used for analysis of SCC.
Three separate dilutions of the samples were used for analysis.
These samples were used for "input" DNA library preparation for the GeCKO libraries.
All eight samples were used for siblings or relatives, she said.
The RNA samples were used for metatranscriptomic analysis in this study.
The average RPKM values of three biological replicates in each sample were used for further analysis.
Rat kidney cortex samples were used for immunohistochemical analyses as described previously34,49.
A total of 500,000 cells per sample were used for both immunoprecipitation and control (IgG, SantaCruz).
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