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Every other week, the PUF sampler was removed and a new sampler and battery attached.
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Residual DNA in the samples was removed using DNase I (Takara).
The sample was removed from the solution prior to measurement.
At time zero, a sample was removed immediately and the remaining samples removed at regular intervals.
They were oven dried at 70 °C until the entire moisture contained within the sample was removed.
The sample was removed only when the temperature was lower than 100°C.
After 4 h, the sample was removed from the furnace and cooled.
To perform Gas Chromatography (GC), asphaltene fraction from the sample was removed with n-hexane.
The biochar of this sample was removed when it reached 950 °C.
After 1 min, the excessive sample was removed with a filter paper.
Once the furnace got cooled to near room temperature, the sample was removed from it.
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