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Whole plant where sampled to avoid the random effects associated with bottleneck colonization of different leafs by different viral subpopulations.
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Bias: Was the population sampled to avoid bias?
Species richness was calculated as the average number of species between the two years of sampling to avoid overestimating the number of species present simultaneously in each patch, and was modeled as a Poisson variable in all cases.
The samples were brought to the lab under cold conditions and processed within 1 h of sampling to avoid artefact damage to the DNA.
Specifically, both of them represent the unknown target distribution by a set of randomly drawn samples to avoid the intractable integrals.
We carried out the test separately on the CEU and YRI samples, to avoid the effects of this population structure on the analysis.
We discarded transcripts for which we did not detect expression in all samples or where expression was close to zero in all samples to avoid the effects of low read sampling error on expression levels (RPKM<1).
In this study, we used OF and DBS samples to avoid the difficulty of blood sampling from infants.
We chose, however, to analyse the more stringent sample to avoid the influence of parity on the main outcome variables.
The other reason is that the definition can balance the positive samples and negative samples to avoid the training bias.
Mice were fasted overnight before administrating these samples to avoid the influence of food in the digestive tracts.
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