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To enrich cDNA libraries for transcripts that represent a greater proportion of early acting factor to later responding regulators, we sampled root tissue at the early-time points of salt stress.
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The sampled roots and soils were transported to Mekelle University laboratory for estimating root colonization and spore extraction.
Jackson and his colleagues descended into the caves and sampled roots of four tree species--juniper, evergreen oak, deciduous oak, and deciduous bumelia lanuginosa, also known as chittamwood.
We sampled roots extensively by careful separation from soil.
We sampled roots and leaves of S. lycopersicum cv.
Fresh and dry weights were measured at two different time points to determine that the sampled roots were in a phase with an ongoing accumulation.
We sampled Jatropha root, stem and leaf tissues from three different germplasm accessions.
Primarily, the classical CTAB (Cetyl trimethylammonium Bromide) method18 was used for genomic DNA extraction from solid samples (root powder, tablet and pill) and ethanol precipitation for liquid samples (oral liquid and injection).
The identity of all CW samples (root and rhizome) was authenticated to be dried using morphological and histological methods by Dr. Lu Zhang.
Yet, according to Mokany et al. [20], sampling to what may be deemed an insufficient soil depth to capture the majority of the roots, while not sampling the root collar or fine roots, as well as sampling with inadequate replication, are a few of the methodological pitfalls associated with sampling root biomass, and can lead to underestimation.
Among all samples, root tissue had the lowest number of transcripts of all PfDGATs.
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