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Four nonredundant cDNAs were isolated from the sampled lens tissues by RT-PCR using RNA and a high-fidelity DNA polymerase (EasyA high-fidelity PCR cloning enzyme; Agilent Technologies Santa Clara, CA, USA).
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In high-resolution optical coherence tomography (OCT), a high numerical aperture sample lens is required to achieve high lateral resolution.
Pressure recordings at the lens are sampled at a frequency of 100 Hz and are transmitted telemetrically to a base unit where the data is stored.
Interestingly, they did not recover any Fusarium isolate through extensive sampling of lens solutions from the patients, or from environmental sampling of the plant where the solution was manufactured.
The measurement of identical proxy journal samples indicated that the light source, the AWB balance on the smartphone and sample to lens distance had the largest effect on L xy output.
Both iPhones were held at distances between 7 and 1 cm at 1 cm intervals in a number of different lighting conditions as follows (1) natural sunlight indoors (2) fluorescent strip lighting of the archive (3) LED lamp at a distance of 10 cm (4) A second iPhone LED light held in a clamp allowed LED light to flood the axis of sample to lens.
Biological samples of lens crystallin proteins obtained from the human patients suffering from diabetes were supplied by the eye clinic Lexum (Wroclaw, Poland).
The light is focused into the sample by lens MO1 and the object, which is mounted on a translation stage, is scanned in three dimensions.
However, this interpretation is limited due to the use of solid sample (intact lens) for analysis, wherein the absorbency may reach a point of saturation at lower wavelengths (blue-end region of the spectra).
and TG(17∶0/17∶0/17∶0) (Larodan Fine Chemicals) was added to the lens samples before homogenization The samples were homogonised, with the HPLC-grade chloroform and methanol (3∶1; 3000 µL).
THz pulses were focused onto a sample by optical lens, and the THz beam carrying sample's information met the probe laser beam at the ZnTe crystal in THz dector [18].
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