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For each site, aquatic sediments were sampled in triplicate from the top layer of the bedded sediments.
A portion of the sludge suspension was sampled in triplicate, and, after being dried at 105 °C for 24 h, they were weighed.
A portion of the mixed sludge suspension was sampled in triplicate, and, after being dried at 105 °C for 24 h, they were weighed.
Multiple colonies (4 10) from each population were sampled in triplicate and five bee midguts were pooled for each sample.
Sample collection was performed over a two-day span in July 2008, with each colony sampled in triplicate and each replicate comprised of midguts from five nurse bees.
Five animals were sampled in triplicate at times 0, 4 and 8 h, rinsed with unlabeled seawater, gently dried with a tissue to remove seawater, and weighed (±0.1 mg).
Similar(46)
The reproducibility (± 5% for most elements) was checked by digesting one sample in triplicate.
For all plasma assays, standard curve samples were run in duplicate and experimental samples in triplicate.
We tested samples in triplicate in parallel with the housekeeping gene GUSB.
In brief, each plate contained the same reference sample in triplicate.
A total of 500 cells were counted for each sample in triplicate.
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