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After fixation in buffered formalin for 12 weeks, the whole brain and the spinal cord were sampled as per our departmental protocol for MND.
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Asphaltenes were extracted from unsonicated and sonicated naphtha diluted bitumen samples as per ASTM-D6560-12.
Different laboratory tests were conducted on the soil samples as per Indian Standard Code of Practice for Testing of Soils.
For oxidative stress detection, CellROX® Green Reagent (ROS) (C10444, Life Technologies, USA) was added to the samples as per the product instructions.
Ovaries were also fixed in FAA and cleared using methyl salicylate in order to examine a greater number of samples, as per Koltunow et al. (2011).
Given SIRE values extracted from these audio samples as per Table 3, we generated 16 movement sequences using a simulated NAO shown on a projected screen.
The stainless steel strips were bonded on the surface of several RC samples using the Externally Bonded Reinforcement (EBR) bonding technique, whilst the rods were bonded into a groove previously made on the surface of the RC samples as per the Near Surface Technique (NSM).
Separate hybridization cocktails were made using 15 micrograms of fragmented cRNA from each sample as per Affymetrix's protocol.
Biotinylated targets suitable for hybridization to the GeneChip probe arrays were prepared from the RNA samples as per 'One-Cycle Target Labeling' protocol (Affymetrix).
Relative intensity values for each hybridization signal were calculated and ranked according to intensity to allow comparison of relative abundance of genes in each functional category across experimental samples as per Yergeau, et al., (2007).
Total RNA was extracted from the stored samples as per Jahn et al. [ 57].
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