Three biological replicates were used to perform an unpaired t test [t test for independent samples with separate variance estimates (two-sided P)].
A nested, multi-stage, sampling with separating distances increasing in geometric progression from stage to stage will do that.
A separate Beadchip was used to perform a technical replica experiment, testing the same pooled RNA sample with two separate arrays and to test pooled lumbar cord samples from G93A SOD1 mutant rats and from WT littermates, at 10 weeks of age, at onset and at end-stage disease.
Test compounds were diluted by transferring each sample with a separate, disposable glass capillary to water in each trap's tub.
The model has demonstrated a high level of validation and accuracy when subjected to internal split-sample validation with separate training and validation data sets.
Each sample was simulated with separate parameter settings estimated from one of the four real human ENCODE samples (e.g. the simulation parameters for A Rep 1 were estimated from HUVEC Rep 1).
We have designed and built an adaptive sample preparation line with separate modules for combustion, molecular sieve handling, CO2 gas cleaning, CO2 storage, and graphitization.
For the quality of life study and women's preference study a representative sub sample is drawn, with separate consent procedure.
Calibration standards (1 to 1000 ng/mL) and quality control (QC) samples were prepared with separate weighing of toceranib reference material (Pfizer, Kalamazoo, MI, 98.1% purity).
After the creation of the multivariate logistic regression models from the SEER patient data set, model validation was performed using split-sample internal validation with separate training and validation data sets.
