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Technical triplicates were performed for each individual sample, with negative controls in duplicate.
In one lung biopsy sample with negative culture results and histopathology suggesting budding yeast cells as well as hyphal elements, which were interpreted as pseudohyphae, sequencing of the amplicon obtained by the 28S-PCR identified A. fumigatus, while the ITS-2 identified C. tropicalis.
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Figure 7 shows a comparison of viable cell densities for samples with negative control after 3 days of culture.
The remaining 39% samples with negative chloro-alkaline index indicated exchange between Ca2+ and/or Mg2+ in groundwater with Na+ and/or K+ in the aquifer mineral.
A check of cDNA production was performed by amplification of the housekeeping gene β2 M using 0.5 μL cDNA samples with negative controls.
NGS enabled the identification of bacteria potentially responsible for urosepsis in three out of four urine samples with negative conventional culture.
It is probable that samples with negative results had been excessively diluted before the IFA.
Reverse transcription was also performed priming with VIF-VPUoutR1 for the samples with negative vif-vpu region amplification results.
As expected, all samples with positive pericarp tests contained ≥60% Bt seeds, while samples with negative pericarps had ≤20% Bt seeds, confirming the test's utility for differentiating between pollen- and seed-mediated gene flow [5].
In addition, the putative presence of PCR inhibitors in O. cf. ovata samples with negative amplification was verified by adding 2 copies of pLSUO plasmid to the 1∶1, 1∶10 and 1∶100 dilutions of crude extracts of environmental samples.
TaqDNA inhibition was checked in all samples with negative results.
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