There were ten HER2+ samples with mutation status and 2-week/pre-Ki67% data; however, the small sample size precluded any meaningful separate analysis of this subgroup.
These mutations (298 in total) involved 262 genes and most were nonsynonymous, with the top mutated genes as EGFR (4 out of 27, 14.8%), TP53, KRAS and RPS6KB2 (all in 3 samples with mutation frequency 11%).
Also, one of the mutation occurred in a sample with low mutation rate, thus we could find it significant.
Given that the switch III region is most frequently mutated across all Ras isoforms, we focused on patient samples with mutations in this region.
To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant 'a' determinant region, which vary by ethnographic region.
The number of cancer samples with mutations in both Genes A and B (AandB), that of cancer samples with mutations only in Gene A (AnotB), that of cancer samples with mutations only in Gene B (BnotA), and that of cancer samples with mutations in neither Gene A nor gene B (notAnotB) were then counted.
Samples with a mutation in c108+c59 or c108+c51 of dhfr but no additional mutations were classified as double mutations; samples with mutations in c51+c59+c108 as triple mutations; samples with mutations in c51+c59+c108 of dhfr and c437 of dhps were considered quadruple mutations; and samples with mutations in c51+c59+c108 of dhfr and c437+c540 of dhps as quintuple mutations.
All samples with mutations had single mutations.
Nevertheless, wild-type sample plots were quite distinct from samples with mutations.
In samples with mutations, it may suggest LOH, which is consistent with the two-hit hypothesis.
