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Thirdly, additional leverage is gained by linking this existing census sample with individual level war time data from a child evacuee registry.
The first approach made use of total RNA to produce a broad range library (BRL) for each sample, with individual sequencing datasets comprising of between 7,543,861 and 11,648,479 reads.
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Individuals with a Short Physical Performance Battery score 8 ≤7 were preferentially enrolled (45% of the sample) to enrich the sample with individuals at high risk for major mobility disability.
In each country, samples consist of a probability household sample, with individuals aged 50 years or older plus their (possibly younger) partners.
Samples with individual matrix anion concentrations of below 125 mg/L would allow the direct injection, and those above this value would need to be prepared accordingly.
As the purpose of this research was to establish that in principle, lignin could be quantitatively determined using NIR spectroscopy, samples with individual fibre colour change (1) or no observable colour change (0) following the phloroglucinol test were grouped together.
However, compared to samples with Ct-values ≤30 this decline was statistically significant among samples with individual Ct-values ≥35 (Table 2).
On the other hand, samples with individuals showing ambiguities that correspond to multiple possible genotypes were allowed because such ambiguities can easily be treated by the Gene[rate] computer programs.
This bleed through was assessed through measurements performed with samples transfected with individual dyes and mathematically removed from the images of FRET.
This leaves us with an effective sample of 15,005, with individual country samples varying from 2,296 for the Netherlands to 360 in Portugal.
"Studying evolution requires large sample sizes with individual-based data covering the entire life span of each born person," said researcher Virpi Lummaa at the University of Sheffield in England.
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