Samples were collected (4th, 8th, 12th, 16th, 20th, 24th, 28th, 32nd, 36th h) and evaporated at room temperature.
The treated samples of every 2nd, 4th, 8th, 12th, 16th, 20th, 24th, and 28th day were taken out for the analysis.
River water was sampled at the 1st, 3rd, 6th, 10th, and 15th days of fish exposure.
Note that since NC percentiles were obtained from a sample with BMI < 85th percentile, the specificity is almost the same as the NC threshold.
Samples with lower 2nd PC have higher expression of inflammatory genes and vice versa.
In this case we determine the 16th sample with no discriminating error.
Of the 718 that were seronegative by Focus HSV-2 ELISA, every 10th sample with sufficient volume (N = 67) was selected to test by Biokit and WB.
The present study was implemented with a sample of 209 5th and 6th grade elementary school students.
In all models we controlled for age (initially categorically, but finally more finely as continuous variable), while in the analysis of pre-menopausal women we further controlled for day of the cycle (as an indicator variable with 1=blood sample collected between 2nd and 11th day and 0=else).
Biomass and medium samples were collected on 4th, 8th, 12th, 16th, 20th, 24th, 28th, 32nd and 40th days by unloading the bioreactor in whole (batch cultivation).
