As heterogeneous methylation can be identified but not quantified on MS-HRM analysis [ 100], two analyses were performed: one for samples with an estimated level of methylation 10% and greater while excluding samples with heterogeneous methylation, and the second analysis included all heterogeneously methylated samples in the same category.
The improved activity for CO oxidation over samples with heterogeneous Pt distribution is likely due to less tendency towards CO self-poisoning through the development of steeper concentration gradients in the Pt containing regions in the porous support material.
The properties of commercial samples with heterogeneous distribution of the substituents and laboratory-made samples, with a more homogeneous distribution, are compared to allow a better understanding of the thermogelation process of methylcelluloses.
To further asses the performance of PAC in samples with heterogeneous (wild-type and mutant) transcript expression, we performed PAC on 14 clinical glioblastoma specimens (selected to contain >70% tumor nuclei) that had genomic amplifications of the EGFR oncogene.
These samples included the 16/18 samples with heterogeneous methylation identified by MS-HRM.
Results with our new assay might be more biologically relevant, especially in patient samples with heterogeneous methylation patterns.
Furthermore, in samples with heterogeneous staining for Tn, we observed a reverse correlation between ERK8 and Tn staining.
In our study, a multiplex PCR assay was used to detect genetic mixtures in samples with heterogeneous sequences.
Including the samples with heterogeneous methylation, the frequency of methylation events for ABO and RUNX3 were similar to the literature although numerically less [ 21, 26].
Similar gene expression results were found in control MG and breast cancer (BC) samples, with heterogeneous levels of GRP-F1, MGP, GGCX, and VKOR and increased expression of OPN and TNF α in cancer cases.
