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We used a linear mixed effect (LME) model to fit the values y = log(a/ b) for each sample, with fixed effects for cell type (myocyte or blood) and disease class (FSHD-affected, nonmanifesting, or control), including interactions between them, and a random effect for family.
The indicated uniform length segments (gap between samples) taken from the text form the sample with fixed window size set at (N_{w} = 10text000) words.
Split variance refers to uncertainty associated with drawing a random split of training and testing sets from all possible splits of a given sample with fixed training-testing ratio.
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To extract fixed samples with detergent, fixed coverslips were incubated for 2 hr in buffer containing 1% Triton X-100 and 0.1% saponin.
With fixed samples, three common embedding alternatives are acrylic, wax, and epoxy.
Under Tetranomial Sampling and Product-Binomial Sampling with row fixed, this measure becomes pi_{Pearce}=P(S|T -P(S|T -PT}).
In the first scenario, at each locus, alleles at the founder population were sampled with a fixed probability value of p = 0.5.
After 3 washes with wash buffer the sample was fixed with 2% glutaraldehyde for 5 minutes before staining with 2% uranyl acetate.
One part of each sample was fixed with formalin, embedded with paraffin wax and kept at room temperature.
One part of each sample was fixed with formalin, embedded with paraffin wax and kept at RT.
After being washed, each sample was fixed with 2% paraformaldehyde solution and flow cytometry analysis was performed with FACSCalibur (BD Biosciences).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com