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Combining five small samples also resulted in less sample variability than one large sample, which increases statistical power to detect changes.
The strength of the present study is that it is based on random population sample, which increases its overall validity.
The results within this cohort were almost identical to those observed for the entire sample, which increases the validity of these data.
High polydispersity of the product of coupling looks apparent from the PDI of the final polymer sample which increases from 1.58 for PAE copolymer to 2.65 for 4-arm PEG-b-PAE.
The discrepancy in findings between the two studies may be explained by the dependency of the oligo dt) capture method on mass spectrometry analysis to identify all enriched proteins from a complex sample, which increases susceptibility for false negatives.
However, the test-retest results may also be due to the small test-retest sample (which increases the statistical error on kappa, and reduces power on the Wilcoxon signed-rank test) and to the fact that many of the questions relate to experiences and attitudes regarding end-of-life issues which may be modified during a 2-week period of thinking about the issues.
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The entrepreneur responses collected during the preliminary study (24) was also added to the sample, which increased the total of the data set to 279.
85% of the CD patients already underwent surgery once at time of first sample, which increased during follow-up to 90% for the other three time points.
Thirdly, we were not able to adjust for the lipid content of the samples, which increases the measurement error of the chemical concentrations.
The highly degraded nature of DNA extracted from historical samples, which increases with age, temperature and water content [ 13, 14], generally restricts PCR amplification to short fragments (< 200 bp) thus limiting the choice of genetic markers.
Debye Bueche analysis of the light scattering data indicates frozen concentration fluctuations within the gel samples, which increase continuously with increasing crosslink density of the hydrogels.
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