The optimal volume of CVL buffer to be used was determined by a series of preliminary sampling which employed variable volumes of buffer (3 10 mL), according with methods previously published [20]; 7 mL was the optimal volume that allowed a good antibody recovery and prevented either excessive viscosity or unnecessary dilution of the sample.
The use of swab samples which employ M4RT as the transport agent for the COBAS Taqman 48 (Roche) diagnostics assay is fortuitous in that the presence of gentamicin is of benefit to chlamydial tissue culture as it helps control contamination and at the correct concentrations does not inhibit chlamydial growth [16].
The two new sampling strategies, which employ random sampling and quasi-random sampling respectively, compute the elementary effects of each factor at the same initial point and with a same step size in the input space.
Exploring other approaches to synthesis (e.g. through large sample studies which employ quantitative and qualitative methods) as well as retrospective studies which draw on research from different disciplines in one policy domain or set of countries.
Data analysis included the use of multiple linear and logistic regressions, which employed the sampling and replicate weights to account for sampling design.
This study, which employed convenience sampling and the single case design, was conceived as an instrumental case study: "Instrumental case studies examine a particular case to provide insight into an issue or refinement of theory.
Empirical Bayes statistics was applied to shrink the probe-wise sample variances toward a common value, which employed moderated t-statistics test and the moderated F-statistic tests analogous to conventional ANOVA except moderation of residual mean squares and residual degrees of freedom between probes.
This is a cross-sectional study, which employed a multi-stage systematic sampling procedure to recruit study participants.
This was an exploratory study, which employed data from a cross-sectional baseline sample of the PWKP Baseline Survey PWKPBSS).
The CBS/Times poll was carried out by a reputable polling organization, S.S.R.S., which employed human beings, rather than software, to survey a decent-sized sample — sixteen hundred people — via landlines and cell phones.
